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Self-digitization chip for quantitative detection of human papillomavirus gene using digital LAMP

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Abstract

Digital nucleic acid amplification and detection methods provide excellent sensitivity and specificity and allow absolute quantification of target nucleic acids. Isothermal methods such as digital loop-mediated isothermal amplification (digital LAMP) have potential for use in rapid disease diagnosis in low-resource settings due to their speed and lack of thermal cycling. We previously developed a self-digitization (SD) chip, a simple microfluidics device that automatically digitizes a sample into an array of nanoliter wells, for use in digital LAMP. In this work, we improve the SD chip design to increase sample loading efficiency, speed, and completeness, and test a range of well volumes and numbers. We demonstrate the diagnostic capability of this platform by applying it to quantifying human papillomavirus 18 gene.

Graphical abstract: Self-digitization chip for quantitative detection of human papillomavirus gene using digital LAMP

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Publication details

The article was received on 08 Nov 2018, accepted on 01 Feb 2019 and first published on 04 Feb 2019


Article type: Paper
DOI: 10.1039/C8LC01223G
Citation: Lab Chip, 2019, Advance Article

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    Self-digitization chip for quantitative detection of human papillomavirus gene using digital LAMP

    J. E. Kreutz, J. Wang, A. M. Sheen, A. M. Thompson, J. P. Staheli, M. R. Dyen, Q. Feng and D. T. Chiu, Lab Chip, 2019, Advance Article , DOI: 10.1039/C8LC01223G

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