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Issue 2, 2019
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Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay

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Abstract

We have developed a microdevice for therapeutic drug monitoring. In this device, dispensing of sample and reagent was accomplished by simple manual operation of a syringe. Moreover, for a simple and rapid measurement, we used cloned enzyme donor immunoassay as a detection principle. These features and the reagent that is enclosed in microdevice beforehand make it possible to complete the facile analysis. In this paper, our model analyte was 1,3-dimethylxanthine (theophylline), a kind of bronchodilator. The fluorescence measurement of theophylline in whole blood was achieved with the limit of detection of 0.73 μg mL−1. This microdevice provides rapid analysis (4 min), requires only a small volume of sample (2 μL) and features simple operation; hence, it is readily applicable to point of care testing.

Graphical abstract: Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay

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Publication details

The article was received on 17 Oct 2018, accepted on 10 Dec 2018 and first published on 11 Dec 2018


Article type: Paper
DOI: 10.1039/C8LC01105B
Citation: Lab Chip, 2019,19, 233-240

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    Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay

    K. Nishiyama, K. Sugiura, N. Kaji, M. Tokeshi and Y. Baba, Lab Chip, 2019, 19, 233
    DOI: 10.1039/C8LC01105B

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