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Issue 2, 2019
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Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation

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Abstract

We report here a novel pumpless, 96-well plate-based platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation. A gravity-driven flow strategy was developed to generate and sustain the flow rate of culture medium within 10% in the platform's 20 culture chambers. The ability of the platform to generate and sustain the medium flow was demonstrated by computational simulation, flow visualization, and ascertaining the previously known effect of flow-induced shear stress on the stimulated osteogenic differentiation of osteoblasts. The high-throughput utility of the platform was demonstrated by in situ cell staining and high content screening of chemosensitivity assays of multiple myeloma and osteoblast co-cultures. Endpoint characterization and data analyses for all 20 culture chambers required less than 1 hour.

Graphical abstract: Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation

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Publication details

The article was received on 21 Aug 2018, accepted on 06 Dec 2018 and first published on 11 Dec 2018


Article type: Paper
DOI: 10.1039/C8LC00872H
Citation: Lab Chip, 2019,19, 254-261

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    Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation

    Z. Chen, S. He, J. Zilberberg and W. Lee, Lab Chip, 2019, 19, 254
    DOI: 10.1039/C8LC00872H

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