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Issue 6, 2019
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Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry

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Abstract

Molecular imaging of targeted large biomolecules has been restricted in SIMS due to the limited number of probes containing SIMS-detectable isotopes. We introduce here new 19F-containing molecules that can be conjugated in a site-specific manner to nanobodies able to recognize fluorescent proteins (FPs) or mouse immunoglobulins (Igs). In this work, we demonstrate that it is possible to use the 19F-nanobodies to reveal the location of several cellular proteins previously tagged with FPs or Igs. This enables specific bio-imaging in SIMS for a vast repertoire of biomolecules, offering new opportunities to study specific structural and functional molecular interactions in biological specimens.

Graphical abstract: Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry

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Supplementary files

Article information


Submitted
31 Mar 2019
Accepted
24 Apr 2019
First published
24 Apr 2019

This article is Open Access

J. Anal. At. Spectrom., 2019,34, 1083-1087
Article type
Communication

Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry

S. Kabatas, P. Agüi-Gonzalez, R. Hinrichs, S. Jähne, F. Opazo, U. Diederichsen, S. O. Rizzoli and N. T. N. Phan, J. Anal. At. Spectrom., 2019, 34, 1083
DOI: 10.1039/C9JA00117D

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