Jump to main content
Jump to site search

Issue 8, 2019
Previous Article Next Article

A quantitative nanoparticle extraction method for microsecond time resolved single-particle ICP-MS data in the presence of a high background

Author affiliations

Abstract

The detection of nanoparticles (NPs) in the presence of a high background (BG) is challenging in single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) and leads to inaccurate quantification. In this study, we report a data processing procedure for the deconvolution of SP-ICP-MS data and its application to quantification of both the Ag NP size distribution (20 to 100 nm Ag NPs) and the concentration of dissolved silver ions (Ag+ up to 7.5 μg L−1) in mixtures using Poisson statistics to determine thresholds to identify the beginning and end of NP signal events. SP-ICP-MS with a microsecond time resolution data acquisition system (μsDAQ) and conventional pneumatic nebulization was used for the detection of Ag NPs in the presence of a significant concentration of ionic BG (107Ag+ up to 1 000 000 cps). In contrast to conventional three times standard deviation of the BG (3 × SDBG) decision criterion (normal distribution), our NP ion cloud extraction mechanism from the μsDAQ is based on setting thresholds to determine the beginning and the end of an ion cloud using Poisson statistics, which is suitable for the low count data. The algorithm was applied here for the flagging and detection of Ag NPs in the presence of Ag+. Critical level (false positive probability was set to 5%) and detection limit (false positive and false negative probabilities were set to 5%) based on Poisson distributions were implemented to determine the thresholds. A range of different sets of NP ion cloud extraction conditions were tested to verify the calculated thresholds and to obtain optimal extraction conditions at different BGs (Ag+ concentration). The method can be universally applied for the detection of different elements with SP-ICP-MS.

Graphical abstract: A quantitative nanoparticle extraction method for microsecond time resolved single-particle ICP-MS data in the presence of a high background

Back to tab navigation

Supplementary files

Article information


Submitted
30 Jan 2019
Accepted
29 May 2019
First published
30 May 2019

This article is Open Access

J. Anal. At. Spectrom., 2019,34, 1571-1580
Article type
Paper

A quantitative nanoparticle extraction method for microsecond time resolved single-particle ICP-MS data in the presence of a high background

D. Mozhayeva and C. Engelhard, J. Anal. At. Spectrom., 2019, 34, 1571 DOI: 10.1039/C9JA00042A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements