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Determination of silver nanoparticles in single cells by microwell trapping and laser ablation ICP-MS determination

Abstract

Silver nanoparticles (AgNPs) are currently one of the most manufactured nanomaterials and have been applied in various consumer products due to their distinctive antimicrobial properties. Because of cell heterogeneity, analysis of AgNPs in single cells after exposure is fundamental for biomedical applications or toxicological assay. Here we developed a high throughput method for single cell analysis by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Single cells were efficiently trapped by a polydimethylsiloxane (PDMS) microwell array. Under the optimal conditions, 60 % microwells contained one single cell and 4% microwells contained two cells or more. The cells in array were regularly distributed and thus could be easily targeted, ablated in a grid pattern, and quantitatively analyzed by LA-ICP-MS with a high throughput. The silver mass in single 16HBE cells exposed to AgNPs showed a log-normal distribution, ranging from 0.80 to 383 fg Ag per cell. The average result from single cells was in good agreement with the result from digestion of 3.0×106 cells. The limit of detection (LOD) and limit of quantification (LOQ) were calculated as 0.2 fg Ag and 0.7 fg Ag, respectively. Because of great potential in single cell analysis, LA-ICP-MS will be widely applied for single cell analysis and offer great benefits for the study of biological effects of metal NPs at a single cell level.

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Publication details

The article was received on 13 Dec 2018, accepted on 11 Feb 2019 and first published on 11 Feb 2019


Article type: Paper
DOI: 10.1039/C8JA00438B
Citation: J. Anal. At. Spectrom., 2019, Accepted Manuscript

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    Determination of silver nanoparticles in single cells by microwell trapping and laser ablation ICP-MS determination

    L. Zheng, Y. Sang, R. Luo, B. Wang, F. Yi, M. Wang and W. Feng, J. Anal. At. Spectrom., 2019, Accepted Manuscript , DOI: 10.1039/C8JA00438B

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