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Issue 20, 2019
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Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography

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Abstract

Herein, the effective use of aqueous biphasic systems (ABS) in Fast Centrifugal Partition Chromatography (FCPC) for the purification of PEGylated cytochrome c conjugates is shown. High recoveries (between 88% and 100%) and purities (∼100%) were obtained. Both the unreacted cytochrome c and solvents may be recovered and reused, thus allowing the design of a sustainable process in a continuous regime for the isolation of bioconjugates. This process allowed the reduction of the complete E-factor and carbon footprint at circa 100% and 67%, respectively, reinforcing the important environmental contribution of recycling units.

Graphical abstract: Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography

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Publication details

The article was received on 01 Apr 2019, accepted on 10 Jul 2019 and first published on 10 Jul 2019


Article type: Communication
DOI: 10.1039/C9GC01063G
Green Chem., 2019,21, 5501-5506

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    Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography

    J. H. P. M. Santos, A. M. Ferreira, M. R. Almeida, P. S. G. N. Quinteiro, A. C. R. V. Dias, J. A. P. Coutinho, M. G. Freire, C. O. Rangel-Yagui and S. P. M. Ventura, Green Chem., 2019, 21, 5501
    DOI: 10.1039/C9GC01063G

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