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Solar-driven biocatalytic C-hydroxylation through direct transfer of photoinduced electrons

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Abstract

Despite the immense potential of P450s, the dependence on the nicotinamide cofactor (NADPH) and NADPH-P450 reductase (CPR) limits their employment in the chemical industry. Here, we present a visible light-driven platform for biocatalytic C-hydroxylation reactions using natural flavin molecules, especially flavin mononucleotide, as a photosensitizer. By employing visible light as a source of energy instead of the nicotinamide cofactor, the bacterial CYP102A1 heme domain was successfully applied for photobiocatalytic C-hydroxylation of 4-nitrophenol and lauric acid – in the absence of NADPH and CPR. We present a proof of concept that the photoactivation of flavins is productively coupled with the direct transfer of photoinduced electrons to the P450 heme iron, achieving photobiocatalytic C-hydroxylation reactions.

Graphical abstract: Solar-driven biocatalytic C-hydroxylation through direct transfer of photoinduced electrons

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Publication details

The article was received on 01 Aug 2018, accepted on 16 Nov 2018 and first published on 17 Nov 2018


Article type: Paper
DOI: 10.1039/C8GC02398K
Citation: Green Chem., 2019, Advance Article
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    Solar-driven biocatalytic C-hydroxylation through direct transfer of photoinduced electrons

    T. Le, J. H. Park, D. S. Choi, G. Lee, W. S. Choi, K. J. Jeong, C. B. Park and C. Yun, Green Chem., 2019, Advance Article , DOI: 10.1039/C8GC02398K

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