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(−)-Epicatechin administration protects kidneys against modifications induced by short-term l-NAME treatment in rats

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Abstract

The aim of this work was to evaluate the protective effects of (−)-epicatechin on the kidneys of NO-deprived rats. Male Sprague Dawley rats were divided into three groups: control (C), receiving water and standard diet; L-NAME (L), receiving a solution of N(ω)-nitro-L-arginine methyl ester (L-NAME) (360 mg l−1 in water) as a beverage and standard diet; and L-NAME-(−)-epicatechin (LE), receiving L-NAME solution as a beverage and standard diet supplemented with (−)-epicatechin (4 g kg−1 diet). The L-group showed altered kidney function parameters, evaluated based on plasma urea and creatinine. In parallel, kidney oxidative stress markers, i.e. superoxide anion production, malondialdehyde content, and 3-nitrotyrosine protein adducts, were significantly increased in the L group. In addition, L-NAME treatment induced modifications in kidney NO bioavailability determinants: increased expression of NOX subunits (p47phox, gp91phox, NOXO1, and NOX4) and lowered NOS activity. (−)-Epicatechin administration restored kidney function parameters, oxidative stress markers, expression of p47phox, gp91phox, and NOX4 and NOS activity to control values. These results indicate that (−)-epicatechin can mitigate NO-mediated impairment of kidney function, in part due to its capacity to modulate NOXs, NOSs, and consequently oxidative stress, and NO bioavailability.

Graphical abstract: (−)-Epicatechin administration protects kidneys against modifications induced by short-term l-NAME treatment in rats

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Article information


Submitted
24 Sep 2019
Accepted
31 Oct 2019
First published
05 Nov 2019

Food Funct., 2020, Advance Article
Article type
Paper

(−)-Epicatechin administration protects kidneys against modifications induced by short-term L-NAME treatment in rats

P. D. Prince, C. G. Fraga and M. Galleano, Food Funct., 2020, Advance Article , DOI: 10.1039/C9FO02234A

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