Stevia residue extract increases intestinal uric acid excretion via interacting with intestinal urate transporters in hyperuricemic mice
Hyperuricemia (HUA), is a metabolic disorder which occur due to overproduction or under excretion of uric acid (UA) and is directly linked to the development of many life-threating diseases. There is a growing interest among many researchers to overcome the encumbrance of HUA because conventional drugs are associated with multiple side effects. Thus, the present project was designed to utilize flavonoids and chlorogenic acids enriched stevia residue extract (STVRE) to combat HUA. The results showed that supplementation of STVRE (200 and 400 mg/kg bw) inhibits XOD enzyme in serum, duodenum, jejunum, and ileum tissues. Moreover, UA level in the STVRE groups were also significantly (p<0.05) decreased in serum, duodenum, jejunum, and ileum (tissues and juice). STVRE improved intestinal morphology and oxidative biomarkers in duodenum, jejunum, and ileum tissues. Protein and mRNA expression of ABCG2 were upregulated whereas GLUT9 was downregulated in STVRE treated groups compared with model control group. The supplementation of STVRE significantly attenuated hyperuricemia, oxidative stress, upregulated ABCG2 and downregulated GLUT9 (protein and mRNA) expression in hyperuricemic mice. The results of our study revealed that by-product of stevia has the potential to combat hyperuricemia and can be used as a functional ingredient in the development of nutraceutical products.