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9t18:1 and 11t18:1 activate the MAPK pathway to regulate the expression of PLA2 and cause inflammation in HUVECs

Abstract

trans Fatty acids (TFAs) have been reported to promote vascular diseases mainly by promoting apoptosis and inflammation of vascular endothelial cells. However, it has been reported in recent years that elaidic acid (9t18:1) and vaccenic acid (11t18:1) may have different effects on vascular health. This study investigated the effects of 9t18:1 and 11t18:1 on human umbilical vein endothelial cells (HUVECs) function and the possible mechanism of inflammation by analyzing the phospholipid composition changes and the relationship between phospholipase A2 (PLA2) and MAPK pathway. Here we found that the effect of 11t18:1 on cell viability, membrane damage and cellular inflammation was significantly lower than 9t18:1 (p<0.05). And 9t18:1 and 11t18:1 had different effects on phospholipid composition. Both 9t18:1 and 11t18:1 significantly increased the protein expression of PLA2. Moreover, MAPK pathway regulated the expression of PLA2, inflammatory cytokines and cyclooxygenase-2 (COX-2), and secretion of prostaglandin E2 (PGE2) in HUVECs induced by 9t18:1 and 11t18:1. In conclusion, 9t18:1 and 11t18:1 activated the MAPK pathway which regulated the expression of PLA2 to cause inflammation in HUVECs.

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Publication details

The article was received on 27 Aug 2019, accepted on 01 Dec 2019 and first published on 02 Dec 2019


Article type: Paper
DOI: 10.1039/C9FO01982K
Food Funct., 2019, Accepted Manuscript

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    9t18:1 and 11t18:1 activate the MAPK pathway to regulate the expression of PLA2 and cause inflammation in HUVECs

    S. Hu, Q. Zou, X. Lv, R. Zhou, X. Niu, C. Weng, F. Chen, Y. Fan, Z. Deng and J. Li, Food Funct., 2019, Accepted Manuscript , DOI: 10.1039/C9FO01982K

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