Jump to main content
Jump to site search


In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)

Abstract

A supercritical extraction plant (with a 6 L extraction cell) was successfully used to obtain ergosterol-enriched extracts from Lentinula edodes (40 ºC, 225 bar, 1-5h, 20 L/h recirculated CO2). Ergosterol (ERG) and the SFE extract (SFE) with the highest ergosterol concentration were also microemulsified and submitted to in vitro digestion to study their ability to displace cholesterol from dietary mixed micelles (DMMs). ERG was also mixed with a β-glucan-enriched (33.5%) extract (BGE) from L. edodes to investigate synergies. Results indicated that they all (including BGE without ERG) could reduce cholesterol levels in DMMs. However, when ERG and SFE were administrated to mice simultaneously with a hypercholesterolemic diet, no significant differences in serum cholesterol levels were detected compared to control. Only when BGE was administrated to another mice model after the hypercholesterolemia was previously induced, significant reduction of cholesterol was noticed.

Back to tab navigation

Publication details

The article was received on 31 Jul 2019, accepted on 07 Oct 2019 and first published on 09 Oct 2019


Article type: Paper
DOI: 10.1039/C9FO01744E
Food Funct., 2019, Accepted Manuscript

  •   Request permissions

    In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)

    D. Morales, E. Tejedor-Calvo, N. Jurado-Chivato, G. Polo, M. Tabernero, A. R. Rodriguez, C. Largo and C. Soler-Rivas, Food Funct., 2019, Accepted Manuscript , DOI: 10.1039/C9FO01744E

Search articles by author

Spotlight

Advertisements