Isolation of strawberry anthocyanin-rich fraction and its mechanisms of action against murine breast cancer cell lines
The aim of this study was the evaluation of the effects of strawberry anthocyanin extracts treatment on two in vitro models of murine breast cancer cell line, trying to detect a specific pathway (AMP-activated protein kinase, or AMPK) through which strawberries exert their anticancer activity. The anticancer activity of anthocyanin purified extracts from Alba cultivar on two murine cancer cell lines, N202/1A (with high levels of HER2/neu oncogene) and N202/1E (with low levels of HER2/neu oncogene), were evaluated after 48 and 72 h of treatment. The cell viability and apoptosis, intracellular ROS rates, and cell oxidative damage were assessed. Western Blot assays were performed to analyze the expression of several proteins related to apoptosis, autophagy, metastasis, oxidative status, mitochondrial functionality, and AMPK pathway. This study demonstrated that the anthocyanin extract of Alba strawberry possessed an antiproliferative effect on cancer cells, through the induction of apoptosis and oxidative stress, by stimulating different molecular pathways. This study is one of the first that have tried to deepen a candidate pathway for the explanation of the strawberry effects on cancer cells. A relation between the AMPK pathway and the anticancer effects of strawberries was demonstrated.