Protective Effect of Pterostilbene on Concanvalin A-Induced Acute Liver Injury
Pterostilbene (PTE) is broadly found in berries with antioxidant and anti-inflammatory properties. To examine the effect of PTE on acute liver injury, mice were administrated with PTE prior to concanavalin A(ConA). Exactly, the mice were divided as follows: (i) vehicle control, (ii) ConA alone, (iii) ConA with PTE at 10 mg/kg (PTE low dose, PTL), and (iv) ConA with PTE at 40 mg/kg (PTE high dose, PTH). After ConA challenge, mice showed prompt induction of intrahepatic INF-γ and TNF-α, followed by tissue factor (TF) which aggravates fibrin deposition and massive liver necrosis whereas these effects were significantly counteracted upon PTE pretreatment. Furthermore, PTE reversed the phosphorylation of ConA-induced intrahepatic inflammatory kinases including JNK, ERK1/2, p38 and p65. Interestingly, PTE didn’t directly act on hepatocytes but inhibit intrahepatic macrophages accumulation and TF generation through inhibiting inflammatory p38 MAPK activation. These results suggest a promising avenue for exploring pterostilbene in improving acute liver injury.