Evaluation of CaD on bone structure and bone metabolic changes in senile osteoporosis rats based on MLP-ANN methods
Senile osteoporosis (SOP) is a related disease of systematic degenerative changes in the body's bones during natural aging. The increasing age is an important factor in its pathogenesis. This experiment was to evaluatin the comprehensive effect of Calcium with Vitamin D3 (CaD) on SOP based on multilayer perceptron (MLP)-artificial neural network methods. 15-month-old male Sprague-Dawley rats were administrated with CaD for 2 months, while 3-, 6-, 9-, 12-, 15- and 17- month-old rats were used as the mature or aging control groups. We detected the bone mass, bone mineral density (BMD), biomechanical testing and micro-CT properties to evaluate the degree of osteoporosis. Levels of alkaline phosphatase (ALP) and Tartrate-resistant acid phosphatase (TRACP), and the ratio of ALP to TRACP both in serum and bone were measured for the evaluation of bone turnover rate. The bone mRNA and protein expression of ATP6v0d2, IGF-1, BMP2, M-CSF, Wnt5a and TGF-β1 were detected by western blotting (WB), immunofluorescence(IF) and quantitative real time polymerase chain reaction (qRT-PCR) for the evaluation of bone metabolism in bone microenvironment. MLP neural network model was constructed and evaluated the importance of related parameters and the comprehensive action of CaD. Our data showed that bone mass, BMD, maximal load, ultimate displacement, ALP and TRACP in serum and tibia, the protein and mRNA expression of ATP6v0d2, IGF-1, BMP2, M-CSF, Wnt5a and TGF-β1 in tibia reached a peak in 6m rats, and then were gradually decreased with the increase of age to the lowest in 17m rats. This study demonstrated that the degeneration of bone structure and bone metabolism in SOP rats during the aging process of rats aged 3 to 17 months. CaD could effectively increase bone mass and bone strength, alleviate the degradation of bone microstructure and rebalance bone remolding. In addition, MLP model was a comprehensive method in evaluating the effects of drugs on SOP, which provided a new direction for future drug and nutrition evaluation.