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Issue 4, 2019
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Immunomodulatory effects of an acidic polysaccharide fraction from herbal Gynostemma pentaphyllum tea in RAW264.7 cells

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Abstract

A new acidic polysaccharide (GPTP-3) with a molecular weight of 2.49 × 106 Da was extracted and purified from Gynostemma pentaphyllum tea. Monosaccharide analysis revealed that GPTP-3 mainly comprised mannose (20.4%), glucuronic acid (17.4%), glucose (33.4%), and galactose (21.4%) (parentheses indicate the molar percentages). Immunostimulating assays indicated that GPTP-3 could markedly promote the secretion of NO, TNF-α, IL-1β, and IL-6 in murine macrophage RAW264.7. TLR4 was found to be a recognized target of GPTP-3. Moreover, TLR4-related mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt, including ERK, JNK, p38, and Akt, were rapidly activated by GPTP-3 in RAW264.7 cells. Furthermore, GPTP-3 was found to induce the nuclear translocation of NF-κB subunit p65. All these findings suggest that MAPK, PI3K/Akt, and NF-κB pathways are involved in GPTP-3-induced macrophage activations, and GPTP-3 has the potential to be developed as a functional food with immunomodulatory functions.

Graphical abstract: Immunomodulatory effects of an acidic polysaccharide fraction from herbal Gynostemma pentaphyllum tea in RAW264.7 cells

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Publication details

The article was received on 31 Jan 2019, accepted on 18 Mar 2019 and first published on 22 Mar 2019


Article type: Paper
DOI: 10.1039/C9FO00219G
Food Funct., 2019,10, 2186-2197

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    Immunomodulatory effects of an acidic polysaccharide fraction from herbal Gynostemma pentaphyllum tea in RAW264.7 cells

    D. Ren, Y. Zhao, Q. Zheng, A. Alim and X. Yang, Food Funct., 2019, 10, 2186
    DOI: 10.1039/C9FO00219G

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