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Potential mechanisms mediating the protective effects of a peptide from walnut (Juglans mandshurica Maxim.) against hydrogen peroxide induced neurotoxicity in PC12 cells

Abstract

Ameliorations of oxidative stress were main approaches to improve neurodegenerative disorder. In the present study, a walnut peptide with strong capacity of scavenging reactive oxygen species (ROS), was purified and identified as EVSGPGLSPN by SEC, RP-HPLC, and HPLC-MS/MS. Treatment with EVSGPGLSPN could significantly (P < 0.05) reduce ROS generation, increase cell viability, and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-px) activities in a dose-dependent manner in hydrogen peroxide induced PC12 cells. Western blot and immunofluorescence analysis showed that EVSGPGLSPN suppressed the expression of IKKβ and p65 to inhibit NF-κB pathway activation, attenuating the neurotoxic cascade by overexpression of IL-1β and TNF-α. Moreover, EVSGPGLSPN inhibited apoptosis by suppressing the expression of cytochrome C, caspase-9, caspase-3, and PARP. Additionally, it also up-regulated the expression of p-CREB and synaptophysin in oxidatively damaged PC12 cells. Thus, EVSGPGLSPN may protect against hydrogen peroxide induced neurotoxicity by enhancing the activity of antioxidant enzymes and blocking the NF-κB/Caspase pathways.

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Publication details

The article was received on 24 Dec 2018, accepted on 07 May 2019 and first published on 08 May 2019


Article type: Paper
DOI: 10.1039/C8FO02557F
Food Funct., 2019, Accepted Manuscript

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    Potential mechanisms mediating the protective effects of a peptide from walnut (Juglans mandshurica Maxim.) against hydrogen peroxide induced neurotoxicity in PC12 cells

    C. Liu, Y. Guo, F. Zhao, H. Qin, H. Lu, L. Fang, J. Wang and W. Min, Food Funct., 2019, Accepted Manuscript , DOI: 10.1039/C8FO02557F

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