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Nutraceutical protein isolate from pigeon pea (Cajanus cajan) milling waste by-product: Functional aspects and digestibility

Abstract

The aim of the present study was to utilize the protein from pigeon pea milling waste by-product for the preparation of protein isolate (PPI). The PPI was exposed to pepsin hydrolysis to reveal functional aspects like increased antioxidant capacity along with gelling ability. Complexation study demonstrated use of PPI as an encapsulating material for water insoluble bioactive compounds (curcumin). Fluorescence spectroscopy revealed the formation of a complex through hydrophobic interaction. Ex-vivo digestibility of PPI’s major proteins resulted in many peptides after digestion with human gastrointestinal enzymes. The peptide sequences identified may have potential biological activities as revealed by bioinformatic data analysis. The presence of bioactive peptides and essential amino acids in the ex-vivo digest suggests the role of PPI as a nutraceutical protein. The study provides a new value addition to the pigeon pea milling waste by-product, as a novel nutraceutical protein which can be used in functional food formulations.

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Publication details

The article was received on 04 Oct 2018, accepted on 12 Apr 2019 and first published on 16 Apr 2019


Article type: Paper
DOI: 10.1039/C8FO01933A
Citation: Food Funct., 2019, Accepted Manuscript

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    Nutraceutical protein isolate from pigeon pea (Cajanus cajan) milling waste by-product: Functional aspects and digestibility

    A. Tapal, G. Vegarud, A. Sreedhara and P. Kaul Tiku, Food Funct., 2019, Accepted Manuscript , DOI: 10.1039/C8FO01933A

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