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Factors contributing to variability of glycan microarray binding profiles

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Abstract

Protein–carbohydrate interactions play significant roles in a wide variety of biological systems. Glycan microarrays are commonly utilized to interrogate the selectivity, sensitivity, and breadth of these complex protein–carbohydrate interactions. During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities. Comparative analyses of glycan microarray data have shown that while many protein–carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results. For optimal construction and use of this technology, it is important to understand sources of variances across array platforms. In this study, we performed a systematic comparison of microarray data from 8 lectins across a range of concentrations on the CFG and neoglycoprotein array platforms. While there was good general agreement on the binding specificity of the lectins on the two arrays, there were some cases of large discrepancies. Differences in glycan density and linker composition contributed significantly to variability. The results provide insights for interpreting microarray data and designing future glycan microarrays.

Graphical abstract: Factors contributing to variability of glycan microarray binding profiles

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Publication details

The article was received on 18 Feb 2019, accepted on 01 Apr 2019 and first published on 24 Jul 2019


Article type: Paper
DOI: 10.1039/C9FD00021F
Faraday Discuss., 2019, Advance Article

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    Factors contributing to variability of glycan microarray binding profiles

    J. S. Temme, C. T. Campbell and J. C. Gildersleeve, Faraday Discuss., 2019, Advance Article , DOI: 10.1039/C9FD00021F

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