Jump to main content
Jump to site search


Poly(alkyl glycidyl ether) hydrogels for harnessing the bioactivity of engineered microbes

Abstract

Herein, we describe a method to produce yeast-laden hydrogel inks for direct-write 3D printing cuboidal lattices for immobilized whole-cell catalysis. A poly(alkyl glycidyl ether)-based triblock copolymer was designed to have three important features for this application: (1) a temperature response which allowed for facile processing of the material; (2) the shear response which facilitated the extrusion of the material through a nozzle; and (3) a UV-light induced polymerization which enabled the post extrusion chemical crosslinking of network chains, and the fabrication of robust printed objects. These three key stimuli responses were confirmed via rheometrical characterization. A genetically-engineered yeast strain with an upregulated α-factor production pathway was incorporated into the hydrogel ink and 3D printed. The immobilized yeast cells exhibited adequate viability of 87.5% within the hydrogel. The production of the up-regulated α-factor was detected using a detecting strain and quantified at 268 nM (s = 34.6 nM) over 72 h. The reusability of these bioreactors was demonstrated by immersion of the yeast-laden hydrogel lattice in fresh SC media and confirmed by the detection of similar amounts of up-regulated α-factor 259 nM (s = 45.1 nM). These yeast-laden materials represent an attractive opportunity for whole-cell catalysis of other high-value molecules in a sustainable and continuous manner.

Back to tab navigation

Supplementary files

Publication details

The article was received on 14 Feb 2019, accepted on 05 Mar 2019 and first published on 06 Mar 2019


Article type: Paper
DOI: 10.1039/C9FD00019D
Faraday Discuss., 2019, Accepted Manuscript

  •   Request permissions

    Poly(alkyl glycidyl ether) hydrogels for harnessing the bioactivity of engineered microbes

    T. G. Johnston, C. R. Fellin, A. Carignano and A. Nelson, Faraday Discuss., 2019, Accepted Manuscript , DOI: 10.1039/C9FD00019D

Search articles by author

Spotlight

Advertisements