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Poly(alkyl glycidyl ether) hydrogels for harnessing the bioactivity of engineered microbes

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Abstract

Herein, we describe a method to produce yeast-laden hydrogel inks for the direct-write 3D printing of cuboidal lattices for immobilized whole-cell catalysis. A poly(alkyl glycidyl ether)-based triblock copolymer was designed to have three important features for this application: (1) a temperature response, which allowed for facile processing of the material; (2) a shear response, which facilitated the extrusion of the material through a nozzle; and (3) UV light induced polymerization, which enabled the post-extrusion chemical crosslinking of network chains, and the fabrication of robust printed objects. These three key stimuli responses were confirmed via rheometrical characterization. A genetically-engineered yeast strain with an upregulated α-factor production pathway was incorporated into the hydrogel ink and 3D printed. The immobilized yeast cells exhibited adequate viability of 87.5% within the hydrogel. The production of the upregulated α-factor was detected using a detecting yeast strain and quantified at 268 nM (s = 34.6 nM) over 72 h. The reusability of these bioreactors was demonstrated via immersion of the yeast-laden hydrogel lattice in fresh SC media and confirmed by the detection of similar amounts of upregulated α-factor at 259 nM (s = 45.1 nM). These yeast-laden materials represent an attractive opportunity for whole-cell catalysis of other high-value products in a sustainable and continuous manner.

Graphical abstract: Poly(alkyl glycidyl ether) hydrogels for harnessing the bioactivity of engineered microbes

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Publication details

The article was received on 14 Feb 2019, accepted on 05 Mar 2019 and first published on 06 Mar 2019


Article type: Paper
DOI: 10.1039/C9FD00019D
Faraday Discuss., 2019, Advance Article

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    Poly(alkyl glycidyl ether) hydrogels for harnessing the bioactivity of engineered microbes

    T. G. Johnston, C. R. Fellin, A. Carignano and A. Nelson, Faraday Discuss., 2019, Advance Article , DOI: 10.1039/C9FD00019D

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