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Biopolymer monolith for protein separation

Abstract

The porous glycopolymers, ”glycomonoliths”, were prepared by radical polymerization based on the polymerization-induced phase separation with acrylamide derivative of -mannose, acrylamide and cross-linker in order to investigate the protein adsorption and separation. The porous structure were induced by porogenic alcohol, and the pore diameter and surface area were controlled by the kinds of alcohol. The protein adsorption was measured in both batch and continuous flow systems. Glycomonolith showed the specific interaction to sugar recognition protein of concanavalin A, and non-specific interaction to other proteins was negligible small. The amount of protein adsorption to materials was determined by the sugar density and the physical property of glycomonoliths. The protein adsorption was also attained by continuous flow system. Fundamental knowledge of glycomonolith for protein separation was obtained.

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Publication details

The article was received on 11 Feb 2019, accepted on 24 Apr 2019 and first published on 25 Apr 2019


Article type: Paper
DOI: 10.1039/C9FD00018F
Faraday Discuss., 2019, Accepted Manuscript

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    Biopolymer monolith for protein separation

    Y. Miura, H. Seto, M. Shibuya and Y. Hoshino, Faraday Discuss., 2019, Accepted Manuscript , DOI: 10.1039/C9FD00018F

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