Biopolymer monolith for protein separation
The porous glycopolymers, ”glycomonoliths”, were prepared by radical polymerization based on the polymerization-induced phase separation with acrylamide derivative of -mannose, acrylamide and cross-linker in order to investigate the protein adsorption and separation. The porous structure were induced by porogenic alcohol, and the pore diameter and surface area were controlled by the kinds of alcohol. The protein adsorption was measured in both batch and continuous flow systems. Glycomonolith showed the specific interaction to sugar recognition protein of concanavalin A, and non-specific interaction to other proteins was negligible small. The amount of protein adsorption to materials was determined by the sugar density and the physical property of glycomonoliths. The protein adsorption was also attained by continuous flow system. Fundamental knowledge of glycomonolith for protein separation was obtained.
- This article is part of the themed collection: Nanolithography of biointerfaces