Jump to main content
Jump to site search


Biopolymer monolith for protein purification

Author affiliations

Abstract

Porous glycopolymers, “glycomonoliths”, were prepared by radical polymerization based on polymerization-induced phase separation with an acrylamide derivative of α-mannose, acrylamide and cross-linker in order to investigate protein adsorption and separation. The porous structure was induced by a porogenic alcohol. The pore diameter and surface area were controlled by the type of alcohol. The protein adsorption was measured in both batch and continuous flow systems. The glycomonoliths showed specific interaction with the sugar recognition protein of concanavalin A, and non-specific interaction to other proteins was negligible. The amount of protein adsorption to the materials was determined by the sugar density and the composition of the glycomonoliths. Fundamental knowledge regarding the glycomonoliths for protein separation was obtained.

Graphical abstract: Biopolymer monolith for protein purification

Back to tab navigation

Publication details

The article was received on 11 Feb 2019, accepted on 24 Apr 2019 and first published on 25 Apr 2019


Article type: Paper
DOI: 10.1039/C9FD00018F
Faraday Discuss., 2019, Advance Article

  •   Request permissions

    Biopolymer monolith for protein purification

    Y. Miura, H. Seto, M. Shibuya and Y. Hoshino, Faraday Discuss., 2019, Advance Article , DOI: 10.1039/C9FD00018F

Search articles by author

Spotlight

Advertisements