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A ‘catch-and-release’ receptor for the cholera toxin

Abstract

Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.

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Publication details

The article was received on 11 Feb 2019, accepted on 20 Mar 2019 and first published on 21 Mar 2019


Article type: Paper
DOI: 10.1039/C9FD00017H
Citation: Faraday Discuss., 2019, Accepted Manuscript

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    A ‘catch-and-release’ receptor for the cholera toxin

    C. Mahon, G. C. Wildsmith, D. Haksar, E. de Poel, R. Pieters, J. M. Beekman, M. Webb and W. B. Turnbull, Faraday Discuss., 2019, Accepted Manuscript , DOI: 10.1039/C9FD00017H

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