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COLLECTION AND IDENTIFICATION OF AN UNKNOWN COMPONENT FROM EUGENIA UNIFLORA ESSENTIAL OIL EXPLOITING A MULTIDIMENSIONAL PREPARATIVE THREE-GC SYSTEM EMPLOYING APOLAR, MID-POLAR AND IONIC LIQUID STATIONARY PHASES

Abstract

The present research deals with the collection and further structure elucidation of an unknown component, accounting for about 35%, from the essential oil obtained after distillation of the leaves of Eugenia Uniflora L., harvested during summer (January, 2017) at Parana State (South of Brazil). A multidimensional gas chromatographic preparative system, based on the coupling of three GCs equipped with apolar, PEG and ionic liquid-based stationary phases, respectively, was successfully applied for the isolation of the chromatographic band relative to the unknown molecule. The use of wide-bore columns allowed an increased sample capacity respect conventional micro bore columns, permitting the injection of a neat sample, greatly reducing the total collection time. The multidimensional approach in heart-cut mode, at the same time has guaranteed a higher chromatographic efficiency, exploiting the different selectivity of the three stationary phases, ensuring the achievement of a high purity fraction. In only five runs, more then 3 milligrams were collected with an average purity greater then 95%. Finally, the unknown component was subjected to nuclear magnetic resonance, mass spectrometry and condensed phase Fourier-Trasform infrared spectroscopies driving to the identification of a 6-ethenyl-6-methyl-3,5-di(prop-1-en-2-yl)cyclohex-2-en-1-one. The presented approach has demonstrated to be effectively for the isolation and structure elucidation of molecules from complex samples for further in deep studies, i.e. for biological evaluation or pharmacological tests.

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Publication details

The article was received on 09 Dec 2018, accepted on 16 Jan 2019 and first published on 16 Jan 2019


Article type: Paper
DOI: 10.1039/C8FD00234G
Citation: Faraday Discuss., 2019, Accepted Manuscript

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    COLLECTION AND IDENTIFICATION OF AN UNKNOWN COMPONENT FROM EUGENIA UNIFLORA ESSENTIAL OIL EXPLOITING A MULTIDIMENSIONAL PREPARATIVE THREE-GC SYSTEM EMPLOYING APOLAR, MID-POLAR AND IONIC LIQUID STATIONARY PHASES

    D. Sciarrone, A. Schepis, G. De Grazia, A. Rotondo, F. Alibrando, H. Bizzo, C. Deschamps, L. M. Sidisky and L. Mondello, Faraday Discuss., 2019, Accepted Manuscript , DOI: 10.1039/C8FD00234G

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