Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 1, 2019
Previous Article Next Article

EMA-amplicon-based taxonomic characterisation of the viable bacterial community present in untreated and SODIS treated roof-harvested rainwater

Author affiliations

Abstract

Illumina next generation sequencing coupled with the viability dye ethidium monoazide bromide (EMA) was utilised to characterise and compare the viable bacterial community present in roof-harvested rainwater pre- and post-treatment. The diversity and relative abundance of the viable bacterial community present in roof-harvested rainwater before (tank 1) and after solar disinfection treatment (SODIS-CPC-1) for eight h, was also determined. Taxonomic assignments were made using the Ribosomal Database Project whereafter alpha- and beta-diversity indices were calculated to investigate the effect of SODIS treatment on the viable bacterial population present in roof-harvested rainwater. Alpha-diversity indices, including species richness and Shannon diversity were significantly (p < 0.05) lower in the SODIS-CPC-1 treated rainwater samples in comparison to tank 1 rainwater samples, indicating a significant difference in the species richness after treatment. The tank 1 rainwater samples were dominated by the families Nocardiaceae (16.5%) and Pseudomonadaceae (8.9%), while the SODIS-CPC-1 rainwater samples were dominated by Nocardiaceae (44.0%) and Micrococcaceae (31.7%). On the genus level, Rhodococcus (17.1%) and Pseudomonas (9.2%) dominated in the tank 1 rainwater samples, while Rhodococcus (48.0%) and Arthrobacter (35.2%) were the most abundant in the SODIS-CPC-1 rainwater samples. While, viable opportunistic pathogenic bacteria such as Pseudomonas and Clostridium XI were detected in both the tank 1 and SODIS-CPC-1 rainwater samples, beta-diversity analysis, using the Bray-Curtis distance metric system, indicated that there was a significant shift (PERMANOVA, p < 0.05) in the viable bacterial community after SODIS treatment. Based on the results obtained, EMA-amplicon-based taxonomic analysis could be utilised as an alternative culture-independent method for the initial monitoring and profiling of the viable and viable-but-non-culturable whole bacterial community in water sources.

Graphical abstract: EMA-amplicon-based taxonomic characterisation of the viable bacterial community present in untreated and SODIS treated roof-harvested rainwater

Back to tab navigation

Supplementary files

Article information


Submitted
04 Sep 2018
Accepted
17 Nov 2018
First published
22 Nov 2018

Environ. Sci.: Water Res. Technol., 2019,5, 91-101
Article type
Paper

EMA-amplicon-based taxonomic characterisation of the viable bacterial community present in untreated and SODIS treated roof-harvested rainwater

A. Strauss, B. Reyneke, M. Waso, T. Ndlovu, C. J. Brink, S. Khan and W. Khan, Environ. Sci.: Water Res. Technol., 2019, 5, 91
DOI: 10.1039/C8EW00613J

Social activity

Search articles by author

Spotlight

Advertisements