Issue 10, 2019

How reversible are the effects of silver nanoparticles on macrophages? A proteomic-instructed view

Abstract

Silver nanoparticles are known to strongly affect biological systems, and numerous toxicological studies have investigated their effects. Most of these studies examine the effects immediately following acute exposure. In this work, we have conducted further investigation by studying not only the acute, post-exposure response, but also the cellular response after a 72 hour-recovery-phase post exposure. As a biological model we have used macrophages, which are very important cells with respect to their role in the immune response to particulate materials. To investigate the response of macrophages to nanoparticles and their recovery post exposure, we have used a combination of proteomics and targeted experiments. These experiments provided evidence that the cellular reaction to nanoparticles, including the reaction during the recovery phase, is a very active process involving massive energy consumption. Pathways such as the oxidative stress response, central and lipid metabolism, protein production and quality control are strongly modulated during the cellular response to nanoparticles, and restoration of basic cellular homeostasis occurs during the recovery period. However, some specialized macrophage functions, such as lipopolysaccharide-induced cytokine and nitric oxide production, did not return to their basal levels even 72 hours post exposure, showing that some effects of silver nanoparticles persist even after exposure has ceased.

Graphical abstract: How reversible are the effects of silver nanoparticles on macrophages? A proteomic-instructed view

Supplementary files

Article information

Article type
Paper
Submitted
08 Apr 2019
Accepted
21 Jul 2019
First published
20 Sep 2019
This article is Open Access
Creative Commons BY-NC license

Environ. Sci.: Nano, 2019,6, 3133-3157

How reversible are the effects of silver nanoparticles on macrophages? A proteomic-instructed view

B. Dalzon, A. Torres, H. Diemer, S. Ravanel, V. Collin-Faure, K. Pernet-Gallay, P. Jouneau, J. Bourguignon, S. Cianférani, M. Carrière, C. Aude-Garcia and T. Rabilloud, Environ. Sci.: Nano, 2019, 6, 3133 DOI: 10.1039/C9EN00408D

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements