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Issue 10, 2019
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How reversible are the effects of silver nanoparticles on macrophages? A proteomic-instructed view

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Abstract

Silver nanoparticles are known to strongly affect biological systems, and numerous toxicological studies have investigated their effects. Most of these studies examine the effects immediately following acute exposure. In this work, we have conducted further investigation by studying not only the acute, post-exposure response, but also the cellular response after a 72 hour-recovery-phase post exposure. As a biological model we have used macrophages, which are very important cells with respect to their role in the immune response to particulate materials. To investigate the response of macrophages to nanoparticles and their recovery post exposure, we have used a combination of proteomics and targeted experiments. These experiments provided evidence that the cellular reaction to nanoparticles, including the reaction during the recovery phase, is a very active process involving massive energy consumption. Pathways such as the oxidative stress response, central and lipid metabolism, protein production and quality control are strongly modulated during the cellular response to nanoparticles, and restoration of basic cellular homeostasis occurs during the recovery period. However, some specialized macrophage functions, such as lipopolysaccharide-induced cytokine and nitric oxide production, did not return to their basal levels even 72 hours post exposure, showing that some effects of silver nanoparticles persist even after exposure has ceased.

Graphical abstract: How reversible are the effects of silver nanoparticles on macrophages? A proteomic-instructed view

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Supplementary files

Article information


Submitted
08 Apr 2019
Accepted
21 Jul 2019
First published
20 Sep 2019

This article is Open Access

Environ. Sci.: Nano, 2019,6, 3133-3157
Article type
Paper

How reversible are the effects of silver nanoparticles on macrophages? A proteomic-instructed view

B. Dalzon, A. Torres, H. Diemer, S. Ravanel, V. Collin-Faure, K. Pernet-Gallay, P. Jouneau, J. Bourguignon, S. Cianférani, M. Carrière, C. Aude-Garcia and T. Rabilloud, Environ. Sci.: Nano, 2019, 6, 3133
DOI: 10.1039/C9EN00408D

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