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Issue 4, 2019
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Protein corona formed on silver nanoparticles in blood plasma is highly selective and resistant to physicochemical changes of the solution

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Abstract

Nanoparticles (NPs) in contact with protein-containing media such as biological fluids rapidly acquire a surface layer of proteins, known as the protein corona. The protein composition and structural properties of the protein corona are crucial for NP interactions with living cells. Although much has been learned about the protein corona phenomenon, further elucidation could benefit from extensive quantitative proteomics analysis. Herein we report a comprehensive quantitative characterization (>350 proteins) of the corona that formed on 60 nm silver NPs via interaction with human blood plasma, as a function of pH and temperature. By varying the pH and temperature one can access different conformational spaces and charge localizations of the plasma proteins, which in turn provide knowledge pertinent to how the proteome corresponds to binding affinity. Thirty-eight percent of the quantified proteins bind at all temperatures, 47% at all pH values, and of these most persistent proteins, approximately 60% do not significantly change in abundance within the protein corona. Evaluation of 544 protein properties (present in the Kyoto databank) suggests that binding of these proteins to NPs is determined by the extent of hydrophobicity, β-sheet propensity, α-helical structure (and turns), and amino acid composition. Protein binding is promoted by a larger amount of β-sheets, higher hydrophobicity, and a smaller amount of α-helices. Our work enhances researchers' knowledge of a long-standing, vexing aspect of the nano–bio interface.

Graphical abstract: Protein corona formed on silver nanoparticles in blood plasma is highly selective and resistant to physicochemical changes of the solution

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Publication details

The article was received on 21 Sep 2018, accepted on 22 Jan 2019 and first published on 18 Mar 2019


Article type: Paper
DOI: 10.1039/C8EN01054D
Environ. Sci.: Nano, 2019,6, 1089-1098
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    Protein corona formed on silver nanoparticles in blood plasma is highly selective and resistant to physicochemical changes of the solution

    V. Gorshkov, J. A. Bubis, E. M. Solovyeva, M. V. Gorshkov and F. Kjeldsen, Environ. Sci.: Nano, 2019, 6, 1089
    DOI: 10.1039/C8EN01054D

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