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Sponge-derived polybrominated diphenyl ethers and dibenzo-p-dioxins, irreversible inhibitors of the bacterial α-d-galactosidase

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Abstract

An integrated in vitro and in silico approach was applied to evaluate the potency of hydroxylated polybrominated diphenyl ethers (OH-PBDEs) and spongiadioxins (OH-PBDDs) isolated from Dysidea sponges on the activity of the recombinant α-D-galactosidase of the GH36 family. It was revealed for the first time that all compounds rapidly and apparently irreversibly inhibited the bacterial α-D-galactosidase. The structure–activity relationship study in the series of OH-PBDEs showed that the presence of an additional hydroxyl group in 5 significantly enhanced the potency (IC50 4.26 μM); the increase of bromination in compounds from 1 to 3 increased their potency (IC50 41.8, 36.0, and 16.0 μM, respectively); the presence of a methoxy group decreased the potency (4, IC50 60.5 μM). Spongiadioxins 6, 7, and 8 (IC50 16.6, 33.1, and 28.6 μM, respectively) exhibited inhibitory action comparable to that of monohydroxylated diphenyl ethers 1–3. Docking analysis revealed that all compounds bind in a pocket close to the catalytic amino acid residues. Molecular docking detected significant compound–enzyme interactions in the binding sites of α-D-galactosidase. Superimposition of the enzyme–substrate and the enzyme–inhibitor complexes showed that their binding sites overlap.

Graphical abstract: Sponge-derived polybrominated diphenyl ethers and dibenzo-p-dioxins, irreversible inhibitors of the bacterial α-d-galactosidase

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Publication details

The article was received on 24 Jun 2019, accepted on 09 Sep 2019 and first published on 10 Sep 2019


Article type: Paper
DOI: 10.1039/C9EM00301K
Environ. Sci.: Processes Impacts, 2019, Advance Article

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    Sponge-derived polybrominated diphenyl ethers and dibenzo-p-dioxins, irreversible inhibitors of the bacterial α-D-galactosidase

    N. K. Utkina, G. N. Likhatskaya, L. A. Balabanova and I. Y. Bakunina, Environ. Sci.: Processes Impacts, 2019, Advance Article , DOI: 10.1039/C9EM00301K

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