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The curious case of opossum prion: a physicochemical study on the copper(II) binding to the bis-decarepeat fragment from the protein N-terminal domain

Abstract

The opossum is a peculiar model of immunity to prion diseases. Here we scrutinised the bis-decarepeat peptide sequence of opossum prion (Op_bis-deca) protein by a multitechnique approach, with a combined experimental (potentiometry, UV-visible, circular dichroism, NMR and EPR spectroscopies, quartz crystal microbalance with dissipation monitoring and confocal microscopy) and simulation (DFT calculations) approach. Results showed that the macrochelate structures formed upon the binding to Cu(II) by the analogous bis-octarepeat peptide sequence of human prion (Hu_bis-octa) are not found in the case of Op_bis-deca. At physiological pH and equimolar amount of copper ions, the [CuLH-2] is the major species formed by Op_bis-deca. In this species one imidazole and two amide nitrogen atoms are involved in metal coordination and its stability constant value is lower compared to the analogous species formed by Hu_bis-octa, due to the presence of an extra proline residue. Moreover, the study on the interaction of the peptides or the peptide/Cu(II) complexes with model cell membranes made of supported lipid bilayers disclosed different levels of interaction, monitored by the viscoelastic changes of the membrane, which exhibited a similar viscoelastic response at the interface with the two complexes, while in the absence of Cu(II), Hu_bis-octa /SLB interface was more viscoelastic than the Op_bis-deca case.

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Publication details

The article was received on 14 Jun 2019, accepted on 04 Nov 2019 and first published on 04 Nov 2019


Article type: Paper
DOI: 10.1039/C9DT02510C
Dalton Trans., 2019, Accepted Manuscript

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    The curious case of opossum prion: a physicochemical study on the copper(II) binding to the bis-decarepeat fragment from the protein N-terminal domain

    A. Magri', G. Tabbì, L. M. Cucci, C. Satriano, A. Pietropaolo, G. Malgieri, C. Isernia and D. La Mendola, Dalton Trans., 2019, Accepted Manuscript , DOI: 10.1039/C9DT02510C

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