Jump to main content
Jump to site search


Tailorable Synthesis of Heterogeneous Enzyme-Copper Nanobiohybrids and application in selective oxidation of benzene to phenol

Abstract

A protein-direct synthetic route to make nanobiohybrids containing copper nanoparticles (CuNPs) using an enzyme in combination with a copper sulfate in aqueous media at room temperature has been described. Enzyme-Cu(ii) intermediates, rapidly generated in aqueous media, are transformed, induced by the enzyme structure, to facilitate the formation of a particular Cu species, tailoring the size of nanoparticles formation depending on the experimental conditions, e.g. pH, reducing step, amount of enzyme or incubation time. This controlled-synthesis allows the preparation of tailor-made nanobiohybrids of Cu(0)NPs, Cu(0)/Cu2ONPs, Cu2ONPs or Cu3(PO4)2 NPs at multimilligram scale. The catalytic performance of the novel CuNPs nanobiohybrids was evaluated in the selective C-H functionalization of benzene by direct monohydroxilation using hydrogen peroxide as green oxidant at mild conditions, aqueous media and moderate temperature. Optimization in the amount of hydrogen peroxide, the presence of co-solvent, temperature was performed, obtaining the production of 17 mM phenol with 83% selectivity using Cu-CALB-PHOS-NR nanobiohybrid as catalyst.

Back to tab navigation

Supplementary files

Publication details

The article was accepted on 26 Nov 2019 and first published on 27 Nov 2019


Article type: Paper
DOI: 10.1039/C9CY02091H
Catal. Sci. Technol., 2019, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Tailorable Synthesis of Heterogeneous Enzyme-Copper Nanobiohybrids and application in selective oxidation of benzene to phenol

    N. Losada-Garcia, A. Rodriguez-Otero and J. M. Palomo, Catal. Sci. Technol., 2019, Accepted Manuscript , DOI: 10.1039/C9CY02091H

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements