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Issue 22, 2019
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Catalytic and biophysical investigation of rhodium hydroformylase

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Rh-Containing artificial metalloenzymes based on two mutants of sterol carrier protein_2L (SCP_2L) have been shown to act as hydroformylases, exhibiting significant activity and unexpectedly high selectivity in the hydroformylation of a range of alkenes. Here we report modifications of the catalyst performance by site directed mutagenesis, and studies of the biophysical properties of these catalysts. Catalysts were prepared based on single methionine mutants of SCP_2L for hydroformylation studies. Multinuclear (1H, & 13C), multi-dimensional (2D) solution NMR spectroscopy, EXAFS and XANES were employed to probe the structure. Biophysical studies using 2D [1H 13C] HSQC NMR spectroscopy of 13C-methyl methionine labeled catalysts were used to investigate changes in protein conformation. Hydroformylation studies employing the methionine mutants as catalysts revealed the significant effects of mutation on hydroformylation activity. Among the methionine mutant catalysts M1A of V83C and A100C, and M112A of V83C exhibited significantly higher activity than their parent proteins with improved selectivity. A metal binding role for methionine was suggested by EXAFS and XANES data on selenomethionine variants.

Graphical abstract: Catalytic and biophysical investigation of rhodium hydroformylase

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Supplementary files

Article information

21 Aug 2019
13 Oct 2019
First published
16 Oct 2019

Catal. Sci. Technol., 2019,9, 6428-6437
Article type

Catalytic and biophysical investigation of rhodium hydroformylase

H. T. Imam, A. G. Jarvis, V. Celorrio, I. Baig, C. C. R. Allen, A. C. Marr and P. C. J. Kamer, Catal. Sci. Technol., 2019, 9, 6428
DOI: 10.1039/C9CY01679A

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