Effect of bovine serum albumin on tartrate-modified manganese ferrite nano hollow spheres: spectroscopic and toxicity study

Abstract

The emerging category of magneto-fluorescent tartrate-modified MnFe₂O₄ nano hollow spheres (T-MnFe₂O₄ NHSs) can be considered as promising candidates for biomedical applications. The interaction of bovine serum albumin (BSA) with T-MnFe₂O₄ NHSs has been studied using several spectroscopic techniques, which suggest that the interaction occurs by an electrostatic mechanism. Furthermore, BSA enhances the charge transfer transition from the tartrate ligand to the metal ions along with the d–d transition of Fe³⁺ ions on NHSs surfaces at different pH. Very strong salt bridge formation occurs between the lysine of the BSA surface and the tartrate in basic medium (pH 10), followed by the acidic (pH 3) and neutral medium (pH 7), respectively. Systematic fluorescence microscopic analysis reveals that BSA significantly enhances the contrast of T-MnFe₂O₄ NHSs in UV and blue light excitation because of the extended charge transfer from BSA to T-MnFe₂O₄ NHSs. Our report demonstrates great potential in the field of nanotechnology and biomedical applications. In vitro toxicity analysis using RAW 264.7 celline and in vivo studies on Wister rats revealed that the T-MnFe₂O₄ NHSs are benign. Furthermore, T-MnFe₂O₄ NHSs also appear to be an antimicrobial agent. Therefore, T-MnFe₂O₄ NHSs can be explored for future therapeutic applications.