Accelerated DNAzyme-based fluorescent nanoprobe for highly sensitive microRNA detection in live cells

Yanan Wu; Hong-Min Meng; Juan Chen; Kemei Jiang; Ran Yang; Yingying Li; Ke Zhang; Lingbo Qu; Xiao-Bing Zhang; Zhaohui Li

doi:10.1039/C9CC08598J

Accelerated DNAzyme-based fluorescent nanoprobe for highly sensitive microRNA detection in live cells†

Yanan Wu,^a Hong-Min Meng,*^a Juan Chen,^a Kemei Jiang,^a Ran Yang,^a Yingying Li,^a Ke Zhang,^b Lingbo Qu,*^a Xiao-Bing Zhang

^c and Zhaohui Li

*^a

Author affiliations

* Corresponding authors

^a Institute of Chemical Biology and Clinical Application at the First Affiliated Hospital, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, College of Chemistry, Zhengzhou University, Zhengzhou 450001, P. R. China
E-mail: zhaohui.li@zzu.edu.cn, qulingbo@zzu.edu.cn, hmmeg2017@zzu.edu.cn

^b Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA

^c College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China

Abstract

By assembling DNAzyme on DNA nanowires through DNA hybridization, we have developed a novel accelerated DNAzyme-based fluorescent nanoprobe for fast, sensitive and selective detection of miRNA. Moreover, the strategy was successfully applied for in situ imaging of miRNA-21 in different cell lines.

Chemical Communications

Accelerated DNAzyme-based fluorescent nanoprobe for highly sensitive microRNA detection in live cells†

Abstract

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Accelerated DNAzyme-based fluorescent nanoprobe for highly sensitive microRNA detection in live cells

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