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In vivo biosynthesis of tyrosine analogs and their concurrent incorporation into a residue-specific manner for enzyme engineering

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Abstract

Herein we report the development of an efficient cellular system for the in vivo biosynthesis of Tyr-analogs and their concurrent incorporation into target proteins by the residue-specific approach. This system makes use of common phenol derivatives and the tyrosine phenol lyase machinery to create various tyrosine analogues that impart desired properties on the target proteins. Biosynthesized 2-fluorotyrosine was incorporated into three industrially important enzymes which resulted in enhanced thermostability.

Graphical abstract: In vivo biosynthesis of tyrosine analogs and their concurrent incorporation into a residue-specific manner for enzyme engineering

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Publication details

The article was received on 04 Nov 2019, accepted on 20 Nov 2019 and first published on 21 Nov 2019


Article type: Communication
DOI: 10.1039/C9CC08503C
Chem. Commun., 2019, Advance Article

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    In vivo biosynthesis of tyrosine analogs and their concurrent incorporation into a residue-specific manner for enzyme engineering

    Y. Won, H. Jeon, A. D. Pagar, M. D. Patil, S. P. Nadarajan, D. T. Flood, P. E. Dawson and H. Yun, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC08503C

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