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Issue 99, 2019
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miR-122 direct detection in human serum by time-gated fluorescence imaging

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Abstract

A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.

Graphical abstract: miR-122 direct detection in human serum by time-gated fluorescence imaging

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Publication details

The article was received on 14 Oct 2019, accepted on 15 Nov 2019 and first published on 27 Nov 2019


Article type: Communication
DOI: 10.1039/C9CC08069D
Chem. Commun., 2019,55, 14958-14961

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    miR-122 direct detection in human serum by time-gated fluorescence imaging

    E. Garcia-Fernandez, M. C. Gonzalez-Garcia, S. Pernagallo, M. J. Ruedas-Rama, M. A. Fara, F. J. López-Delgado, J. W. Dear, H. Ilyine, C. Ress, J. J. Díaz-Mochón and A. Orte, Chem. Commun., 2019, 55, 14958
    DOI: 10.1039/C9CC08069D

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