Jump to main content
Jump to site search


miR-122 direct detection in human serum by time-gated fluorescence imaging

Author affiliations

Abstract

A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.

Graphical abstract: miR-122 direct detection in human serum by time-gated fluorescence imaging

Back to tab navigation

Supplementary files

Publication details

The article was received on 14 Oct 2019, accepted on 15 Nov 2019 and first published on 27 Nov 2019


Article type: Communication
DOI: 10.1039/C9CC08069D
Chem. Commun., 2019, Advance Article

  •   Request permissions

    miR-122 direct detection in human serum by time-gated fluorescence imaging

    E. Garcia-Fernandez, M. C. Gonzalez-Garcia, S. Pernagallo, M. J. Ruedas-Rama, M. A. Fara, F. J. López-Delgado, J. W. Dear, H. Ilyine, C. Ress, J. J. Díaz-Mochón and A. Orte, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC08069D

Search articles by author

Spotlight

Advertisements