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Abstract | Cited by

Reported herein is a novel p-quinone methide-based self-immobilizing fluorogenic probe for the visualization of β-galactosidase activities in live cells. This easily prepared imaging reagent massively increases the fluorescence intensity and covalently links to the activation site with high efficiency upon enzymatic trigger.

Graphical abstract: Late-stage difluoromethylation leading to a self-immobilizing fluorogenic probe for the visualization of enzyme activities in live cells

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