Issue 90, 2019
From the journal:

Chemical Communications

Determining transaminase activity in bacterial libraries by time-lapse imaging†

Carlos J. C. Rodrigues, ORCID logo a   João M. Sanches ORCID logo b  and  Carla C. C. R. de Carvalho ORCID logo *a  

* Corresponding authors

a iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal
E-mail: ccarvalho@tecnico.ulisboa.pt

b Institute for Systems and Robotics (ISR/IST), LARSyS, Bioengineering Department, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal

Abstract

Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. A correlation between the benzaldehyde conversion and relative luminance was determined, allowing the identification of the most promising biocatalysts, the determination of kinetic parameters, and the assessment of the effect of the substrate concentration on activity.

Graphical abstract: Determining transaminase activity in bacterial libraries by time-lapse imaging

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Article information

DOI
https://doi.org/10.1039/C9CC07507K
Article type
Communication
Submitted
24 Sep 2019
Accepted
15 Oct 2019
First published
15 Oct 2019

Chem. Commun., 2019,55, 13538-13541

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Determining transaminase activity in bacterial libraries by time-lapse imaging

C. J. C. Rodrigues, J. M. Sanches and C. C. C. R. de Carvalho, Chem. Commun., 2019, 55, 13538 DOI: 10.1039/C9CC07507K

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