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Pyrococcus furiosus Argonaute-mediated nucleic acid detection

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Abstract

We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.

Graphical abstract: Pyrococcus furiosus Argonaute-mediated nucleic acid detection

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Publication details

The article was received on 18 Sep 2019, accepted on 01 Oct 2019 and first published on 02 Oct 2019


Article type: Communication
DOI: 10.1039/C9CC07339F
Chem. Commun., 2019, Advance Article

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    Pyrococcus furiosus Argonaute-mediated nucleic acid detection

    R. He, L. Wang, F. Wang, W. Li, Y. Liu, A. Li, Y. Wang, W. Mao, C. Zhai and L. Ma, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC07339F

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