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Issue 98, 2019
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Chemo-biological mRNA imaging with single nucleotide specificity

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Abstract

Unambiguous imaging of C → U edited mRNA calls for a method that distinguishes a locally high concentration of unbound probe or single nucleotide mismatched target from a locally low concentration of matched mRNA target. To address this issue, we combine FIT probes as a “chemical” detection system with the “biological” MS2 technique. Ratio measurements provide a convenient parameter to discriminate the edited from the unedited state of mRNA encoding for GlyR α2 in HEK cells.

Graphical abstract: Chemo-biological mRNA imaging with single nucleotide specificity

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Publication details

The article was received on 07 Sep 2019, accepted on 13 Nov 2019 and first published on 15 Nov 2019


Article type: Communication
DOI: 10.1039/C9CC06989E
Chem. Commun., 2019,55, 14817-14820

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    Chemo-biological mRNA imaging with single nucleotide specificity

    A. Knoll, S. Kankowski, S. Schöllkopf, J. C. Meier and O. Seitz, Chem. Commun., 2019, 55, 14817
    DOI: 10.1039/C9CC06989E

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