Enhanced in situ detection and imaging of lipids in biological tissues by using 2,3-dicyanohydroquinone as a novel matrix for positive-ion MALDI-MS imaging

Yaqin Liu; Lulu Chen; Liang Qin; Manman Han; Jinming Li; Feixian Luo; Kun Xue; Jinchao Feng; Yijun Zhou; Xiaodong Wang

doi:10.1039/C9CC06961E

Enhanced in situ detection and imaging of lipids in biological tissues by using 2,3-dicyanohydroquinone as a novel matrix for positive-ion MALDI-MS imaging†

Yaqin Liu,‡^ab Lulu Chen,‡^b Liang Qin,‡^b Manman Han,^ab Jinming Li,^ab Feixian Luo,^b Kun Xue,^b Jinchao Feng,^b Yijun Zhou^b and Xiaodong Wang

*^ab

Author affiliations

* Corresponding authors

^a Centre for Imaging & Systems Biology, Minzu University of China, Beijing 100081, China
E-mail: Xiaodong@muc.edu.cn

^b College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China

Abstract

A fluorescent dye, 2,3-dicyanohydroquinone (DCH), was optimized for use as a new matrix for positive-ion MALDI-MS imaging, and it provided enhanced lipid detection and imaging in biological tissues. The properties of DCH, include a strong ultraviolet absorption, low volatility in a high-vacuum (∼10⁻⁷ mbar) source, super chemical stability, μm-sized matrix crystals, uniform matrix deposition, and high ionization efficiency for the detection of lipids.

Chemical Communications

Enhanced in situ detection and imaging of lipids in biological tissues by using 2,3-dicyanohydroquinone as a novel matrix for positive-ion MALDI-MS imaging†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Enhanced in situ detection and imaging of lipids in biological tissues by using 2,3-dicyanohydroquinone as a novel matrix for positive-ion MALDI-MS imaging

Social activity

Search articles by author

Spotlight

Advertisements