Issue 94, 2019
From the journal:

Chemical Communications

Highly specific and sensitive detection of microRNAs by tandem signal amplification based on duplex-specific nuclease and strand displacement†

Qing Liu,a   Peng-Jian Kang,a   Zeng-Ping Chen, ORCID logo *a   Cai-Xia Shi,a   Yao Chenb  and  Ru-Qin Yu ORCID logo a  

* Corresponding authors

a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
E-mail: zpchen@hnu.edu.cn

b Hunan Key Lab of Biomedical Materials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou, P. R. China

Abstract

Based on the full understanding of the hydrolysis patterns of duplex-specific nuclease (DSN) against the probe DNAs in DNA/microRNA heteroduplexes, a simple and generic platform for highly specific and sensitive detection of microRNAs was developed by seamlessly integrating DSN-assisted target recycling amplification and strand displacement amplification in tandem.

Graphical abstract: Highly specific and sensitive detection of microRNAs by tandem signal amplification based on duplex-specific nuclease and strand displacement

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C9CC06790F
Article type
Communication
Submitted
01 Sep 2019
Accepted
03 Nov 2019
First published
04 Nov 2019

Chem. Commun., 2019,55, 14210-14213

Permissions

Request permissions

Highly specific and sensitive detection of microRNAs by tandem signal amplification based on duplex-specific nuclease and strand displacement

Q. Liu, P. Kang, Z. Chen, C. Shi, Y. Chen and R. Yu, Chem. Commun., 2019, 55, 14210 DOI: 10.1039/C9CC06790F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements