Jump to main content
Jump to site search


Highly specific and sensitive detection of microRNAs by tandem signal amplification based on duplex-specific nuclease and strand displacement

Author affiliations

Abstract

Based on the full understanding of the hydrolysis patterns of duplex-specific nuclease (DSN) against the probe DNAs in DNA/microRNA heteroduplexes, a simple and generic platform for highly specific and sensitive detection of microRNAs was developed by seamlessly integrating DSN-assisted target recycling amplification and strand displacement amplification in tandem.

Graphical abstract: Highly specific and sensitive detection of microRNAs by tandem signal amplification based on duplex-specific nuclease and strand displacement

Back to tab navigation

Supplementary files

Publication details

The article was received on 01 Sep 2019, accepted on 03 Nov 2019 and first published on 04 Nov 2019


Article type: Communication
DOI: 10.1039/C9CC06790F
Chem. Commun., 2019, Advance Article

  •   Request permissions

    Highly specific and sensitive detection of microRNAs by tandem signal amplification based on duplex-specific nuclease and strand displacement

    Q. Liu, P. Kang, Z. Chen, C. Shi, Y. Chen and R. Yu, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC06790F

Search articles by author

Spotlight

Advertisements