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19F NMR studies on γ-butyrobetaine hydroxylase provide mechanistic insights and suggest a dual inhibition mode

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Abstract

The final step in the biosynthesis of L-carnitine in humans is catalysed by the 2-oxoglutarate and ferrous iron dependent oxygenase, γ-butyrobetaine hydroxylase (BBOX). 1H and 19F NMR studies inform on the BBOX mechanism including by providing evidence for cooperativity between monomers in substrate/some inhibitor binding. The value of the 19F NMR methods is demonstrated by their use in the design of new BBOX inhibitors.

Graphical abstract: 19F NMR studies on γ-butyrobetaine hydroxylase provide mechanistic insights and suggest a dual inhibition mode

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Publication details

The article was received on 20 Aug 2019, accepted on 23 Sep 2019 and first published on 08 Nov 2019


Article type: Communication
DOI: 10.1039/C9CC06466D
Chem. Commun., 2019, Advance Article
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    19 F NMR studies on γ-butyrobetaine hydroxylase provide mechanistic insights and suggest a dual inhibition mode

    R. K. Leśniak, A. M. Rydzik, J. J. A. G. Kamps, A. Kahn, T. D. W. Claridge and C. J. Schofield, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC06466D

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