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Bio-orthogonal chemistry-based method for fluorescent labelling of ribosomal RNA in live mammalian cells

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Abstract

A bio-orthogonal chemistry-based approach for fluorescent labelling of ribosomal RNA is described. It involves an adenosine analogue modified with trans-cyclooctene and masked 5′-phosphate group using aryl phosphoramidate. The incorporation into rRNA has been confirmed using agarose gel electrophoresis, as well as a highly sensitive UHPLC-MS/MS method. Fluorescent labelling of rRNA has been achieved in live HeLa cells via an inverse electron demand Diels–Alder reaction with a tetrazine conjugated to an Oregon Green fluorophore. This communication describes the stepwise approach that led to the development and characterization of the probe. The results demonstrate a new strategy towards development of future fluorescent probes to investigate the biochemistry of nucleic acids.

Graphical abstract: Bio-orthogonal chemistry-based method for fluorescent labelling of ribosomal RNA in live mammalian cells

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Publication details

The article was received on 16 Jul 2019, accepted on 07 Aug 2019 and first published on 07 Aug 2019


Article type: Communication
DOI: 10.1039/C9CC05346H
Chem. Commun., 2019, Advance Article

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    Bio-orthogonal chemistry-based method for fluorescent labelling of ribosomal RNA in live mammalian cells

    K. Wu, M. He, I. Khan, P. N. Asare Okai, Q. Lin, G. Fuchs and M. Royzen, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC05346H

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