Michihiko Tsushima, Shinichi Sato, Tatsuya Niwa, Hideki Taguchi and Hiroyuki Nakamura
Chem. Commun., 2019,55, 13275-13278
DOI:
10.1039/C9CC05231C,
Communication
Magnetic affinity beads functionalized with lactose and ruthenium/dcbpy complexes were developed. Using MAUra, a catalyst-proximity labelling reagent, the catalytic labeling of lactose-binding proteins was achieved with high selectivity on the beads. The first unbiased identification of cellular endogenous lectins bound to lactose (galectin-1 and galectin-3) was achieved with chemical labelling on the affinity beads.
