Jump to main content
Jump to site search


Freezing promoted hybridization of very short DNA oligonucleotides

Author affiliations

Abstract

Shorter DNA probes provide better specificity for hybridization, but they may not form stable duplexes at room temperature. In this study, we used thiazole orange to follow DNA hybridization upon freezing and achieved stable 5-mer duplex DNA. Using multiple short probes in tandem, long DNA could also be studied. This study provides insights into DNA hybridization in the frozen state and expands the application of freezing for nucleic acid chemistry.

Graphical abstract: Freezing promoted hybridization of very short DNA oligonucleotides

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Jun 2019, accepted on 01 Aug 2019 and first published on 05 Aug 2019


Article type: Communication
DOI: 10.1039/C9CC04608A
Chem. Commun., 2019, Advance Article

  •   Request permissions

    Freezing promoted hybridization of very short DNA oligonucleotides

    Y. Xu, K. Huang, A. Lopez, W. Xu and J. Liu, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC04608A

Search articles by author

Spotlight

Advertisements