Sensitive discrimination of single nucleotide variants using a PDA microtube waveguide platform with heterogeneous CHA amplification and competitive inhibition strategy

Abstract

Here, we present a novel PDA microtube waveguide platform by combining heterogeneous CHA amplification with competitive inhibition. The heterogeneous CHA amplification system can preferentially amplify the signal of the correct target, while the competitive inhibition system will preferentially bind with the mutant targets and inactivate their signal.