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Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification

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Abstract

We develop a new fluorescent method for sensitive detection of long noncoding RNAs (lncRNAs) on the basis of duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification. This method exhibits high sensitivity with a detection limit of 0.081 fM, and it can accurately detect the endogenous lncRNA HOTAIR in cancer cells, providing a new approach to study the physiological function of lncRNAs in human diseases.

Graphical abstract: Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification

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Publication details

The article was received on 16 Apr 2019, accepted on 13 May 2019 and first published on 13 May 2019


Article type: Communication
DOI: 10.1039/C9CC02939G
Chem. Commun., 2019, Advance Article

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    Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification

    Y. Zhang, X. Wang, X. Su and C. Zhang, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC02939G

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