A novel fluorescent assay for the ultrasensitive detection of miRNA-21 with the use of G-quadruplex structures as an immobilization material for a signal indicator

Ze-Zhou Yang; Zhi-Bin Wen; Xin Peng; Ya-Qin Chai; Wen-Bin Liang; Ruo Yuan

doi:10.1039/C9CC01850F

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A novel fluorescent assay for the ultrasensitive detection of miRNA-21 with the use of G-quadruplex structures as an immobilization material for a signal indicator†

Ze-Zhou Yang,

^a Zhi-Bin Wen,

^a Xin Peng,

^a Ya-Qin Chai,

^a Wen-Bin Liang

*^a and Ruo Yuan

*^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
E-mail: wenbinliangasu@gmail.com, yuanruo@swu.edu.cn
Fax: +86-23-68253172
Tel: +86-23-68252277

Abstract

In this work, a novel fluorescent assay was proposed for the ultrasensitive detection of microRNA-21 (miRNA-21) based on the efficient immobilization of protoporphyrin IX (PPIX) as signal indicators in massive G-quadruplex structures obtained by target recycling, three-dimensional DNA walker and a rolling circle amplification (RCA) coupled cascade nucleic acid amplification strategy.

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Article information

DOI: https://doi.org/10.1039/C9CC01850F
Article type: Communication
Submitted: 06 Mar 2019
Accepted: 01 May 2019
First published: 02 May 2019

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Chem. Commun., 2019,55, 6453-6456

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A novel fluorescent assay for the ultrasensitive detection of miRNA-21 with the use of G-quadruplex structures as an immobilization material for a signal indicator

Z. Yang, Z. Wen, X. Peng, Y. Chai, W. Liang and R. Yuan, Chem. Commun., 2019, 55, 6453 DOI: 10.1039/C9CC01850F

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Chemical Communications

A novel fluorescent assay for the ultrasensitive detection of miRNA-21 with the use of G-quadruplex structures as an immobilization material for a signal indicator†

Abstract

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A novel fluorescent assay for the ultrasensitive detection of miRNA-21 with the use of G-quadruplex structures as an immobilization material for a signal indicator

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