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Issue 33, 2019
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Expanding the genetic code with a lysine derivative bearing an enzymatically removable phenylacetyl group

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Abstract

We report the genetically encoded incorporation of phenylacetyl protected lysine (PacK) into proteins in Escherichia coli. This unnatural side-chain modification can be enzymatically removed using either penicillin G acylase (PGA) or, surprisingly, the sirtuin SrtN from Bacillus subtilis. Our approach expands the toolbox to reversibly control protein structure and function under very mild and non-denaturing conditions, as demonstrated by triggering the activity of the nonribosomal peptide synthetase GrsA.

Graphical abstract: Expanding the genetic code with a lysine derivative bearing an enzymatically removable phenylacetyl group

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Publication details

The article was received on 18 Jan 2019, accepted on 29 Mar 2019 and first published on 29 Mar 2019


Article type: Communication
DOI: 10.1039/C9CC00475K
Citation: Chem. Commun., 2019,55, 4793-4796

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    Expanding the genetic code with a lysine derivative bearing an enzymatically removable phenylacetyl group

    M. Reille-Seroussi, S. V. Mayer, W. Dörner, K. Lang and H. D. Mootz, Chem. Commun., 2019, 55, 4793
    DOI: 10.1039/C9CC00475K

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