Jump to main content
Jump to site search


Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit

Author affiliations

Abstract

We have proposed a novel strategy for miRNA detection through enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits. Unlike general MB-based miRNA detection based on the one-to-one (1 : 1) hybridization between MBs and miRNA, our system consists of four species of MBs (MBs A, B, C and D) (MB circuits) and is activated by a hybridization chain reaction. MBs stably coexist as hairpin structures that hardly show fluorescence signals in the absence of target miRNA. After miRNA detection, this MB circuit is able to generate fluorescence signals and amplify the fluorescence signal, contributing to improvement in detection sensitivity under iso-thermal conditions without an enzyme. Furthermore, in vitro and in vivo studies have proven that MB circuits can detect low levels of miRNA with high sensitivity, compared to when only one MB alone is used. Therefore, the MB circuits can provide a useful platform for target miRNA detection.

Graphical abstract: Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Jan 2019, accepted on 31 Jan 2019 and first published on 31 Jan 2019


Article type: Communication
DOI: 10.1039/C9CC00351G
Citation: Chem. Commun., 2019, Advance Article

  •   Request permissions

    Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit

    K. Guk, S. G. Hwang, J. Lim, H. Son, Y. Choi, Y. Huh, T. Kang, J. Jung and E. Lim, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC00351G

Search articles by author

Spotlight

Advertisements